Isolation and Purification of Endotoxin by Hydrolytic Enzymes
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چکیده
منابع مشابه
Isolation and purification of endotoxin by hydrolytic enzymes.
Various commercial hydrolases were used in an attempt to degrade the endotoxic lipopolysaccharide macromolecule. Some inert components, such as peptides and nucleic acids, could be removed from endotoxin preparations. As a result, endotoxic activity, measured by pyrogenicity, Shwartzman reaction, and mouse lethality, was increased. The remarkable resistance of endotoxin to hydrolases led to the...
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Certain strains of Moraxella bovis produce tissue-damaging enzymes which may initiate or potentiate infectious bovine keratoconjunctivitis. Thirteen reference strains of this species were characterized physiologically and screened for production of various enzymes by some conventional biochemical tests and the API ZYM system (Analytab Products, Plainview, N.Y.). All 13 strains were hemolytic. A...
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A moderate halophilic bacterium was isolated from the brine samples of Sambhar salt lake, Rajasthan (India). The isolate formed pigmented colonies and the bacterial cells are motile, Coccoid, Gram positive, strictly aerobic, catalaseand oxidasepositive, ferments carbohydrates, while failed to utilize citrate as sole carbon source. The isolate grew in presence of 2-20% (w/v) NaCl and at pH 7-11,...
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It has been shown that several hydrolytic enzymes with different specificities undergo similar reactions with diisopropyl phosphorofluoridate (1) and other related toxic organophosphorus compounds (2). These conclusions were based on kinetic studies, particularly the influence of pH on the rates of reaction, and on inhibition characteristics. Reactions of this type have provided a unique method...
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The most recent developments in the use of hydrolytic enzymes proteases, lipases and nitrilases are summarized: Sterically demanding a,a-disubstituted carboxylic esters can be enzymatically resolved using a commercially available protease derived from Aspergillus oryzae. A significant enhancement of selectivity and activity of Candida cylindracea lipase in irreversible acyl transfer reactions m...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1972
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.6.6.928-933.1972